Reparative and immonoregulatory effects of porcine dermal biopolymer hydrolysate in wound healing

Biopolymer materials similar to extracellular matrix (ECM) can imitate natural cellular environment, stimulating cell migration and proliferation. Evaluation of reparative and anti-inflammatory effects of ECM hydrolyzed biopolymers can elucidate the role of various growth factors and interleukins at different healing stages. We examined dynamics of ECM components (fibronectin, collagen type I and IV, cytokeratin), PDGF, VEGF, TGFβ1, IGF1, interleukins IL1β, IL6, IL10 in rat blood serum during skin wound treatment with hydrolyzed dermal biopolymer hydrogel. The hydrogel promoted adhesion, migration and proliferation of fibroblasts and increased their viability and metabolic activity during joint incubation in vitro. It also optimized inflammation, angiogenesis and re-epithelialization. In the experimental group, PDGF synthesis was suppressed during the first five days after wounding, IGF1 was enhanced from day 7, and VEGF and TGFβ1 were induced on day 1 and 7, respectively. We observed an increase in IL1β at the start of the experiment, and variations in IL6 and IL10 values on different days. These results will clarify the role of hydrolyzed collagen in ECM restoration and wound-related inflammation, providing a basis for developing hydrogel xenodermal mimetics.