Right Ventricle of Patients Undergoing Congenital Cardiac Surgery Differentially Expresses Haem Oxygenase-1 and Heat Shock Protein 70 Genes

Q: What is the clinical evidence from this study?

Study design: Observational. Population: Congenital heart disease with cardiac hypertrophy (n=8). Intervention: Congenital heart disease / cardiac hypertrophy. Primary outcome: mRNA expression levels of haem oxygenase-1 (HO-1) and heat shock protein 70 (HSP70) in right ventricular muscle.

Q: What does this research mean for the field?

Hypertrophic right ventricular cardiac muscle in pediatric patients undergoing congenital cardiac surgery exhibits significantly increased and differentially regulated mRNA levels of haem oxygenase-1 and heat shock protein 70. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Mamoru Takeuchi; Takashi Takahashi; Naoyuki Taga; Tsutomu Iwasaki; Hiroko Shimizu; Hideki Nakatsuka; S Sano; Reiko Akagi; Kiyoshi Morita

doi:10.1177/147323000303100509

October 1, 2003Journal of International Medical Research

Right Ventricle of Patients Undergoing Congenital Cardiac Surgery Differentially Expresses Haem Oxygenase-1 and Heat Shock Protein 70 Genes

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Key result

Right ventricular hypertrophy in pediatric heart surgery linked to increased HO-1 and HSP70 expression in ~100% of specimens.

n=8

Why the study?

Reactive oxygen species are implicated in cardiac hypertrophy, and haem oxygenase-1 (HO-1) is induced by oxidative stress to protect against tissue injury, motivating examination of HO-1 and heat shock protein 70 (HSP70) expression in hypertrophic myocardium.

Key Points

To evaluate the gene expression of haem oxygenase-1 (HO-1) and heat shock protein 70 (HSP70) in hypertrophic right ventricular myocardium from pediatric patients with congenital heart defects.
Obtained hypertrophic cardiac muscle tissue specimens from eight patients (one infant and seven children) undergoing corrective surgery for congenital heart disease.
Assessed mRNA expression levels of HO-1 and HSP70 across all tissue specimens using Northern blot analysis.
Levels of HO-1 and HSP70 mRNA were significantly increased across all eight hypertrophic cardiac muscle specimens.
The orders of magnitude of gene expression increases differed between HO-1 and HSP70, indicating that the two genes are regulated separately under oxidative stress.

Study Design

Type

Observational (n=8)

Structured PICO

P

Population

8 pediatric patients (one infant and seven children) with hypertrophic cardiac muscle undergoing surgery for congenital heart disease.

E

Exposure

Northern blotting analysis of right ventricular hypertrophic cardiac muscle specimens

O

Outcome

Expression levels of haem oxygenase-1 (HO-1) and heat shock protein 70 (HSP70) mRNAsurrogate

Hypertrophic right ventricular myocardium in pediatric congenital heart disease patients exhibits significant, separately regulated upregulation of stress-response genes HO-1 and HSP70.

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Implication

Potential protective role of HO-1 in pediatric cardiac hypertrophy is hypothesis-generating; leaves open any clinical relevance in congenital heart disease.

Abstract

Reactive oxygen species are implicated in the pathogenesis of cardiac hypertrophy. Haem oxygenase-1 (HO-1), the rate-limiting enzyme in haem catabolism, is induced by oxidative stress and confers protection against oxidative tissue injuries. We used Northern blotting to examine expression of HO-1 and heat shock protein 70 (HSP70) in the hypertrophic cardiac muscle of eight patients (one infant and seven children) who underwent surgery for congenital heart disease. Levels of HO-1 and HSP70 mRNA were significantly increased in all specimens, but the orders of magnitude of the increases were different, suggesting that the genes expressing HO-1 and HSP70 are regulated separately.

Discussion

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Cite This Study

Takeuchi et al. (2003) conducted an observational in Congenital heart disease with cardiac hypertrophy (n=8). Congenital heart disease / cardiac hypertrophy was evaluated on mRNA expression levels of haem oxygenase-1 (HO-1) and heat shock protein 70 (HSP70) in right ventricular muscle. Hypertrophic right ventricular muscle in 8 children undergoing congenital cardiac surgery showed significantly increased HO-1 and HSP70 mRNA in 100% (8/8) of specimens.

synapsesocial.com/papers/6ae7d7242b84c27d3edcc6bd https://doi.org/10.1177/147323000303100509

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Right Ventricle of Patients Undergoing Congenital Cardiac Surgery Differentially Expresses Haem Oxygenase-1 and Heat Shock Protein 70 Genes

Key Points

Study Design

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Right Ventricle of Patients Undergoing Congenital Cardiac Surgery Differentially Expresses Haem Oxygenase-1 and Heat Shock Protein 70 Genes

Key Points

Study Design

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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