Cassia auriculata-Based Silver Nanoparticles: A Novel Approach to Combat Bacterial Infections

Background: Bioactive compounds extracted from Cassia auriculata, a plant with therapeutic uses, promote the reduction of Ag+ to AgNPs.By avoiding the use of potentially hazardous substances typically employed in chemical synthesis, the green synthesis methodology offers a more economically viable and sustainable way of manufacturing nanoparticles.Methods: AgNPs were generated through the addition of an extract from C. auriculata leaves with a solution of silver nitrates.The dimension and form of the nanoparticles have been investigated using X-ray diffraction (XRD), ZETA Potential, Fourier Transform Infra-Red (FTIR), UV-VIS spectroscopy, and scanning electron microscopy (SEM) to validate the synthesis.By the agar well diffusion method, the antibacterial property of the generated AgNPs was measured against B. cereus, P. aeruginosa, S. aureus, and E. coli.Results: AgNPs of C. auriculata were confirmed by UV-VIS spectroscopy analysis with a peak around 421nm.The antibacterial tests showed significant inhibition zones for all tested bacterial strains, with S.aureus exhibiting the highest sensitivity.Considerable restricting zones appeared for E. coli, P. aeruginosa, and B. cereus, demonstrating the AgNPs' broad-spectrum antibacterial effectiveness.Conclusions: Effective antibacterial activity over Gram-positive, as well as Gram-negative bacteria, has been shown by the generated AgNPs from C. auriculata leaf extract.The variances in cell wall construction among different kinds of bacteria may be the root cause of the diversity in the inhibitory zones.The study underscores the potential of using plant-based synthesis to produce AgNPs with strong antibacterial effects.