Structural characterization and phylogenetic analysis of the chloroplast genome of Monarda didyma (Lamiaceae)

Monarda didyma is an aromatic herb native to eastern North America with ecological, medicinal, and ornamental value, but its chloroplast genome has not been characterized. Here, we assembled its complete chloroplast genome (151,812 bp) using next-generation data. The genome exhibits a typical quadripartite structure (LSC: 83,025 bp; SSC: 17,663 bp; IRs: 25,562 bp each), contains 132 genes (87 protein-coding, 37 tRNA, 8 rRNA genes) and 37.82% GC content. Phylogenomic analysis resolved M. didyma and Pycnanthemum tenuifolium as a fully supported sister clade (BS = 100%). This genome provides a resource for species identification and phylogenetic studies of Monarda and related genera.