Genomic DNA extraction and Nanopore sequencing of three Delftia strains v1

This workflow links the MB 360 adaptation of the NO-MISS extraction method to rapid-barcoded MinION sequencing. It was used for Delftia tsuruhatensis ATCC BAA-554 and D. acidovorans ATCC 13751 and ATCC 15668. The study generated eight strain-specific Nanopore libraries across three flow cells. The extraction and kit references below supply the reagent quantities, incubation times, and loading instructions.