Key result
Novel dissociation protocol yields multicellular cardiac cultures from single neonatal mouse hearts for sex-specific analysis.
Why the study?
This protocol was adapted to generate primary cardiac cell cultures from single neonatal mouse hearts that retain cardiomyocytes along with endogenous fibroblast and endothelial cells, enabling genotype- and sex-specific analysis.
This adapted protocol enables the generation of primary cardiac cell cultures retaining cardiomyocytes, fibroblasts, and endothelial cells from single neonatal mouse hearts for genotype- and sex-specific preclinical analyses.
Answers are grounded in this paper and cite their sources.
Enables genotype- and sex-specific cardiac cultures from single neonatal hearts; leaves open validation for disease modeling before research adoption.
Here, we present an adapted primary cardiac cell culture protocol (from Ehler et al. 1 1. Ehler, E. ∙ Moore-Morris, T. ∙ Lange, S. Isolation and Culture of Neonatal Mouse Cardiomyocytes J. Vis. Exp. 2013; 50154 , 50154 Crossref Scopus (194) Google Scholar ) to generate cardiac cultures from single neonatal mouse hearts that retain cardiomyocytes together with endogenous fibroblast and endothelial cell populations. The production of cultures from individual animals enables genotype- and sex-specific analysis. We describe the optimized enzymatic dissociation of an individual heart and culture conditions that support cardiomyocyte maintenance. The sex of each sample is determined using a previously established PCR-based genotyping assay. 2 2. Tunster, S.J. Genetic sex determination of mice by simplex PCR Biol. Sex Differ. 2017; 8 :31 Crossref Scopus (81) PubMed Google Scholar
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York et al. (2026) studied this question. Adapted primary cardiac cell culture protocol was evaluated on Generation of cardiac cultures retaining cardiomyocytes, fibroblasts, and endothelial cells for genotype- and sex-specific analysis. An adapted enzymatic dissociation protocol generates primary cardiac cultures from single neonatal mouse hearts, retaining cardiomyocytes, fibroblasts, and endothelial cells for sex-specific analysis.
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