The AOP-KB Sustainability Plan: A Community Roadmap for the Continued Evolution of the AOP Knowledge Base

The Adverse Outcome Pathway Knowledge Base (AOP-KB), with the AOP-Wiki at its centre, is an internationally recognised resource for developing and sharing mechanistic knowledge that supports New Approach Methodologies (NAMs) and chemical safety assessment. After more than 13 years of community-driven development, securing its future requires coordinated action to maintain the current platform while preparing for the next stage of the AOP-KB. This poster presents the AOP-KB Sustainability Plan as a community roadmap. It outlines a transition that preserves AOP-Wiki 2.x as the authoritative platform while a next-generation AOP-KB 3.0 Core is developed. The proposed ecosystem combines this authoritative Core with a federated layer of specialised capabilities and independent third-party tools, supported by shared principles for interoperability, governance and long-term stewardship. The Plan translates this direction into six Calls for Action: demonstrate the AOP-KB’s value and impact; secure continuity of AOP-Wiki operations; finance and procure the 3.0 Core; safeguard institutional memory and human capacity; develop a participation package; and strengthen stewardship and ecosystem coordination. The poster invites organisations and individuals to identify where their expertise, resources or commitment could help turn these actions into practice. The poster was presented at the 15th Annual Meeting of the American Society for Cellular and Computational Toxicology (ASCCT), held in Durham, North Carolina, USA, on 27–29 October 2026, where it received the Best Poster Award.