Formulation and Invitro Evaluation of Gentamicin Transfersomal Gel

The present study was aimed at the formulation and evaluation of Gentamicin-loaded transferosomal gel for enhanced topical drug delivery. Transferosomes were developed to improve drug penetration and therapeutic efficacy. Preformulation studies including organoleptic evaluation, solubility studies, calibration curve preparation, and FTIR compatibility studies were carried out. Transferosomes were prepared by the thin-film hydration method using soya lecithin, cholesterol, and Span 80, followed by incorporation into Carbopol 940 gel. The prepared formulations were evaluated for particle size, pH, entrapment efficiency, SEM analysis, in vitro drug release, and stability studies.The FTIR studies confirmed the compatibility of Gentamicin with the selected excipients. The particle size of the prepared transferosomes ranged from 112 to 137 nm. SEM analysis revealed spherical vesicles with smooth surface morphology and no signs of aggregation. The pH of all formulations was found to be suitable for topical application. Entrapment efficiency ranged from 79.63% to 85.78%, with formulation F4 exhibiting the highest entrapment efficiency. In vitro drug release studies demonstrated sustained drug release, and formulation F4 showed the highest cumulative drug release of 98.84% after 8 hours. Stability studies indicated that the optimized formulation remained stable during the storage period without significant changes in its physicochemical properties. The results suggest that the developed Gentamicin-loaded transferosomal gel is a promising topical drug delivery system with enhanced drug entrapment, sustained release, and good stability. The optimized formulation may provide improved therapeutic efficacy for the management of topical bacterial infections.