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A new procedure has been devised for the preparation of pure epidermal growth factor in high yield. The method involves a two-step column fractionation of crude extracts of the submaxillary gland obtained from adult male mice. Biologically active epidermal growth factor lacking the COOH-terminal Leu-Arg residues was also isolated.
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C. Richard Savage
Boehringer Ingelheim (Germany)
Stanley Cohen
Rutgers, The State University of New Jersey
Journal of Biological Chemistry
Vanderbilt University
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Savage et al. (Fri,) studied this question.
synapsesocial.com/papers/6a0ccf1f17d56fa39a4a50ae — DOI: https://doi.org/10.1016/s0021-9258(19)44568-7
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