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Laboratory study demonstrates high-yield purification of epidermal growth factor in mice, revealing a biologically active truncated derivative.
A new procedure has been devised for the preparation of pure epidermal growth factor in high yield. The method involves a two-step column fractionation of crude extracts of the submaxillary gland obtained from adult male mice. Biologically active epidermal growth factor lacking the COOH-terminal Leu-Arg residues was also isolated.
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Savage et al. (1972) studied this question.
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