Angiotensin II binding sites were demonstrated in human mononuclear leucocytes, showing rapid, reversible binding with affinity constants comparable to other main target tissues.
This study provides basic science evidence for the presence of specific Angiotensin II binding sites on human mononuclear leucocytes.
Angiotensin II binding sites were demonstrated in human mononuclear leucocytes by use of 125Iangiotensin II. The binding of 125Iangiotensin II to mononuclear leucocytes was rapid and reversible. The abilities of unlabeled compounds to displace 125Iangiotensin II were proportional to their abilities to displace labeled hormone in adrenal and smooth muscle membrane preparations. The Scatchard plot revealed two apparent orders of binding sites. The affinity constants were comparable with those for binding sites in other main target tissues of angiotensin II.
Shimada et al. (Sun,) reported a other. [125I]angiotensin II vs. unlabeled compounds was evaluated on Binding characteristics of [125I]angiotensin II to mononuclear leucocytes. Angiotensin II binding sites were demonstrated in human mononuclear leucocytes, showing rapid, reversible binding with affinity constants comparable to other main target tissues.