The use of acetylethyleneimine in the production of inactivated foot-and-mouth disease vaccines

Suspensions of FMD virus treated with 0·05% formalin at 26° C. for periods up to 144 hr. remained infective for cattle, although the infectivity could not be detected in the presence of aluminium hydroxide. Infectivity was detected in similar virus suspensions which had been treated with 0·05% AEI at 37° C. for 8 hr. but not in suspensions treated for 12 hr. Vaccines prepared from these suspensions were antigenically potent and serum neutralization tests demonstrated the development and regression of serum antibody. The AEI vaccines were at least as potent as the corresponding formalin vaccines.