Effect of Cimetidine on Eggshell Quality and Plasma 25-Hydroxycholecalciferol in Laying Hens

Experiments were conducted to investigate the effect of feeding cimetidine (CIMET), ranging from 0 to 750 mg/kg, on vitamin D3 metabolism and eggshell calcification in laying hens fed two levels of vitamin D3 (500 and 2,000 ICU/kg). Final BW and feed intake were not significantly affected by either CIMET or vitamin D3 level. Feeding 500 and 750 mg of CIMET significantly decreased total egg production in hens fed either level of vitamin D3, but no differences were observed at lower CIMET levels. Tibia ash decreased significantly in hens fed 150 to 750 mg of CIMET, regardless of the vitamin D3 level. Plasma Ca and inorganic P concentrations were decreased in hens fed high CIMET levels (500 and 750 mg/kg) at Week 2, but no differences were observed at Week 4. Feeding CIMET (500 and 750 mg/kg) significantly decreased plasma 25-hydroxycholecalciferol (25-OHD3) levels at Week 2 in hens fed both vitamin D3 diets but not at Week 4. Eggshell breaking force, shell thickness, and percentage shell weight were decreased significantly by CIMET in all experiments; however, in one experiment, shell quality recovered by Week 8. These results suggest that the CIMET-induced reduction in bone mineralization, eggshell quality, and plasma 25-OHD3 levels could be due to interference of CIMET with vitamin D3 metabolism in vitamin D3-replete laying hens. Shell quality decreased in CIMET-treated hens fed the higher vitamin D diet even though 250-HD3 plasma levels were three times higher than in hens fed the lower vitamin D diet, suggesting that CIMET affected shell quality through some mechanism other than inhibition of 250-HD3 synthesis.