Enhancement of Renal but Not Haemodynamic Effects of Atrial Natriuretic Peptide (1–28) in Sheep Treated With Acth

The haemodynamic and renal effects of short-term infusion of human atrial natriuretic peptide (ANP) (1-28) were examined in sheep treated with ACTH and compared with the responses previously observed in normotensive sheep. 2. Infusion of ANP at 100 micrograms/h for 60 min in ACTH-treated sheep (5 micrograms/kg per day for 5 days) decreased blood pressure and produced a fall in both cardiac output and stroke volume. No changes were seen in heart rate and total peripheral resistance. 3. ANP produced large increases in urine volume, urinary sodium and chloride excretion, and further decreased plasma potassium concentration in the ACTH-treated sheep. Compared with normal sheep studied previously under the same conditions, the ACTH-treated sheep showed a much greater diuretic and natriuretic response to ANP, although the blood pressure response to ANP was similar in both states. 4. The change in renal responsiveness to ANP in sheep may be related to the increased blood volume of the ACTH-treated animals because volume expansion is known to enhance the renal effects of ANP.