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We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the specificity threshold and other primer properties. This tool is publicly available at http://www.ncbi.nlm.nih.gov/tools/primer-blast.
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Jian Ye
George Coulouris
Irena Zaretskaya
BMC Bioinformatics
SHILAP Revista de lepidopterología
National Institutes of Health
National Center for Biotechnology Information
Duke-NUS Medical School
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Ye et al. (Mon,) studied this question.
www.synapsesocial.com/papers/69daec4a498b35d3e6a3c01c — DOI: https://doi.org/10.1186/1471-2105-13-134