Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.

Key Points

• The aim is to present a method for preparing standardized suspensions of monkey kidney cells using trypsin.
• Adaptation of Dulbecco's method for trypsin-dispersed cells.
• Quantitative procedures employed for cell suspension preparation.
• Yielding large numbers of standardized replicate cultures.
• Successfully developed a standardized method for cell suspensions.
• Enabled the preparation of multiple replicate cultures efficiently.