Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.

Julius S. Youngner

doi:10.3181/00379727-85-20830

February 1, 1954Experimental Biology and Medicine

Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.

View at Publisher

Ask AI

Bookmark

Share

Key Points

The aim is to present a method for preparing standardized suspensions of monkey kidney cells using trypsin.
Adaptation of Dulbecco's method for trypsin-dispersed cells.
Quantitative procedures employed for cell suspension preparation.
Yielding large numbers of standardized replicate cultures.
Successfully developed a standardized method for cell suspensions.
Enabled the preparation of multiple replicate cultures efficiently.

Ask about this paper

Answers are grounded in this paper and cite their sources.

Implication

Study standardizes cell suspensions in monkey kidney cells, indicating a method for culture preparation.

Abstract

SummaryAn adaptation of the method of Dulbecco for preparation of trypsin-dispersed monkey kidney cell suspensions has been described. Procedures employed yield quantitatively standardized cell suspensions which can be used in the preparation of large numbers of replicate cultures.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Julius S. Youngner (1954) studied this question.

synapsesocial.com/papers/6a1d1a63dabf5784132f3ce1 https://doi.org/10.3181/00379727-85-20830

View at Publisher

Ask AI

Bookmark

Share

Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Monolayer Tissue Cultures I. Preparation and Standardization of Suspensions of Trypsin-Dispersed Monkey Kidney Cells.

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading