Immunohistochemical analysis of human ocular tissues, including 2 Alzheimer's disease cases, revealed widespread distribution of ACE, AngII, and AT1 receptors with no differences from controls.
Observational
The widespread localization of renin-angiotensin system components in the human eye suggests generalized regulatory functions on neuronal cells, vessels, and vitreous humor homeostasis.
We investigated the immunohistochemical distribution of 3 components of the renin-angiotensin system (RAS), angiotensin-converting enzyme (ACE), angiotensin II (AngII) and AT1 receptor (AT1), in the human eye. ACE and AngII were localized to nonpigmented epithelial cells of the ciliary body, to endothelial and epithelial cells of the cornea, to epithelial cells of the conjunctiva and to trabecular meshwork cells in the anterior part of the eye. In the posterior part of the eye, ACE and AngII were localized to ganglion cells, some cells in the inner nuclear layer, photoreceptor cells and to endothelial cells of the retinal and choroidal vessels. The overall intensity of AT1 immunoreactivity was weak in all ocular tissues, but the main localization was in ganglion cells. As a preliminary investigation, we were able to include 2 Alzheimer's disease (AD) cases. In AD, no differences from controls were found in the cellular distribution and staining intensity of all 3 antigens. The manifold localization sites of the observed antigens point to rather generalized functions of the RAS in human ocular tissues, such as regulatory effects on neuronal cells, vessels and vitreous humor homeostasis.
Savaskan et al. (Thu,) conducted a observational in Normal and Alzheimer's disease ocular tissues. Alzheimer's disease vs. Controls was evaluated on Cellular distribution and staining intensity of ACE, AngII, and AT1. Immunohistochemical analysis of human ocular tissues, including 2 Alzheimer's disease cases, revealed widespread distribution of ACE, AngII, and AT1 receptors with no differences from controls.