Oxidative stress with 0.1 mM hydrogen peroxide induced early afterdepolarization-mediated ventricular tachycardia and fibrillation in 94% of aged rat hearts, whereas young adult hearts exhibited no arrhythmias.
Does oxidative stress with hydrogen peroxide induce ventricular arrhythmias more readily in aged hearts compared to young hearts?
Oxidative stress induces ventricular fibrillation and tachycardia in aged hearts, facilitated by intermediate levels of age-related fibrosis.
Absolute Event Rate: 94% vs 0%
Oxidative stress with hydrogen peroxide (H(2)O(2)) readily promotes early afterdepolarizations (EADs) and triggered activity (TA) in isolated rat and rabbit ventricular myocytes. Here we examined the effects of H(2)O(2) on arrhythmias in intact Langendorff rat and rabbit hearts using dual-membrane voltage and intracellular calcium optical mapping and glass microelectrode recordings. Young adult rat (3-5 mo, N = 25) and rabbit (3-5 mo, N = 6) hearts exhibited no arrhythmias when perfused with H(2)O(2) (0.1-2 mM) for up to 3 h. However, in 33 out of 35 (94%) aged (24-26 mo) rat hearts, 0.1 mM H(2)O(2) caused EAD-mediated TA, leading to ventricular tachycardia (VT) and fibrillation (VF). Aged rabbits (life span, 8-12 yr) were not available, but 4 of 10 middle-aged rabbits (3-5 yr) developed EADs, TA, VT, and VF. These arrhythmias were suppressed by the reducing agent N-acetylcysteine (2 mM) and CaMKII inhibitor KN-93 (1 microM) but not by its inactive form (KN-92, 1 microM). There were no significant differences between action potential duration (APD) or APD restitution slope before or after H(2)O(2) in aged or young adult rat hearts. In histological sections, however, trichrome staining revealed that aged rat hearts exhibited extensive fibrosis, ranging from 10-90%; middle-aged rabbit hearts had less fibrosis (5-35%), whereas young adult rat and rabbit hearts had <4% fibrosis. In aged rat hearts, EADs and TA arose most frequently (70%) from the left ventricular base where fibrosis was intermediate ( approximately 30%). Computer simulations in two-dimensional tissue incorporating variable degrees of fibrosis showed that intermediate (but not mild or severe) fibrosis promoted EADs and TA. We conclude that in aged ventricles exposed to oxidative stress, fibrosis facilitates the ability of cellular EADs to emerge and generate TA, VT, and VF at the tissue level.
Morita et al. (Fri,) conducted a other in Ventricular fibrillation susceptibility (n=76). Hydrogen peroxide (H2O2) vs. Young adult hearts was evaluated on Incidence of ventricular tachycardia (VT) and ventricular fibrillation (VF). Oxidative stress with 0.1 mM hydrogen peroxide induced early afterdepolarization-mediated ventricular tachycardia and fibrillation in 94% of aged rat hearts, whereas young adult hearts exhibited no arrhythmias.