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Growth rates and rates of nitrate uptake by N-depleted cells were measured for an oceanic diatom, Chaetoceros gracilis, and a neritic diatom, Asterionella japonica, as functions of nitrate concentration of the medium. Both growth and N-uptake rates appeared to be hyperbolic with nitrate concentration and could be fit to an equation of Michaelis-Menten form: where v is rate, V(m) . is the maximum rate, S is nitrate concentration, and Ks is the half-saturation constant. Ks values for uptake and growth were similar if not identical for each species. Uptake experiments can provide a presumptive measure of Ks for growth, thought to be an ecologically significant characteristic of a species.
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Richard W. Eppley
University of California, Riverside
William H. Thomas
Journal of Phycology
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Eppley et al. (Mon,) studied this question.
synapsesocial.com/papers/69d9de230d540cafc5837d70 — DOI: https://doi.org/10.1111/j.1529-8817.1969.tb02628.x