Demonstrates the in vivo functional importance of the actin-ABP 67 interaction and illustrates the use of suppressor analysis to identify physically interacting proteins.
The protein encoded by SAC6, a gene that can mutate to suppress a temperature-sensitive defect in the yeast actin gene, has been identified as a 67-kilodalton actin-binding protein (ABP 67) that associates with all identifiable actin structures. This finding demonstrates the in vivo functional importance of the actin-ABP 67 interaction previously established in vitro and illustrates the use of suppressor analysis to identify physically interacting proteins.
Adams et al. (Fri,) studied this question.