A new analytical protocol for direct measurement of HDL cholesterol successfully eliminates apoE-rich particles, showing no significant difference compared to the heparin-MnCl2 precipitation method.
Does a new analytical protocol for direct measurement of HDL cholesterol eliminate apoE-rich particles compared to existing methods?
A new analytical protocol enables direct measurement of HDL cholesterol while eliminating apoE-rich particles, aligning more closely with the heparin-MnCl(2) precipitation method than existing direct methods.
It has been reported that the existing direct method of high density lipoprotein (HDL) cholesterol measures particles enriched with apolipoprotein E (apoE). The aim of our study was to investigate a new analytical protocol to directly measure HDL cholesterol that eliminates apoE-rich particles. The interactions of four lipoproteins (HDL(3), HDL(2), LDL, and VLDL + chylomicron) with surfactants, divalent cations, sugars, and lectins were investigated. By analyzing sera, HDL(3), and HDL(2), we examined the relationships among the measurements obtained by our protocol, a precipitation method using heparin-MnCl(2), and a commercially available kit for this direct method. A significant difference was found between the direct method and the heparin-MnCl(2) method, but not between our protocol and the heparin-MnCl(2) method. Multiple regression analysis showed that the difference between the direct method and the heparin MnCl(2) method is dependent on sources of apoE-rich HDL. In conclusion, our protocol enables a direct measurement of HDL cholesterol that eliminates apoE-rich particles.
Okada et al. (Mon,) reported a other. New analytical protocol to directly measure HDL cholesterol vs. Precipitation method using heparin-MnCl2 and a commercially available direct method kit was evaluated on Difference in HDL cholesterol measurements among methods. A new analytical protocol for direct measurement of HDL cholesterol successfully eliminates apoE-rich particles, showing no significant difference compared to the heparin-MnCl2 precipitation method.