Key result
A new analytical protocol for direct measurement of HDL cholesterol successfully eliminates apoE-rich particles, showing no significant difference compared to the heparin-MnCl2 precipitation method.
Why the study?
Does a new analytical protocol for direct measurement of HDL cholesterol eliminate apoE-rich particles compared to existing methods?
Does a new analytical protocol for direct measurement of HDL cholesterol eliminate apoE-rich particles compared to existing methods?
A new analytical protocol enables direct measurement of HDL cholesterol while eliminating apoE-rich particles, aligning more closely with the heparin-MnCl(2) precipitation method than existing direct methods.
May refine direct HDL-C assays in research; leaves open human validation before clinical adoption.
It has been reported that the existing direct method of high density lipoprotein (HDL) cholesterol measures particles enriched with apolipoprotein E (apoE). The aim of our study was to investigate a new analytical protocol to directly measure HDL cholesterol that eliminates apoE-rich particles. The interactions of four lipoproteins (HDL(3), HDL(2), LDL, and VLDL + chylomicron) with surfactants, divalent cations, sugars, and lectins were investigated. By analyzing sera, HDL(3), and HDL(2), we examined the relationships among the measurements obtained by our protocol, a precipitation method using heparin-MnCl(2), and a commercially available kit for this direct method. A significant difference was found between the direct method and the heparin-MnCl(2) method, but not between our protocol and the heparin-MnCl(2) method. Multiple regression analysis showed that the difference between the direct method and the heparin MnCl(2) method is dependent on sources of apoE-rich HDL. In conclusion, our protocol enables a direct measurement of HDL cholesterol that eliminates apoE-rich particles.
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Okada et al. (2001) studied this question. New analytical protocol to directly measure HDL cholesterol vs. Precipitation method using heparin-MnCl2 and a commercially available direct method kit was evaluated on Difference in HDL cholesterol measurements among methods. A new analytical protocol for direct measurement of HDL cholesterol successfully eliminates apoE-rich particles, showing no significant difference compared to the heparin-MnCl2 precipitation method.
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