November 18, 1999Clinical Chemistry and Laboratory Medicine (CCLM)
A New Liquid Homogeneous Assay for HDL Cholesterol Determination Evaluated in Seven Laboratories in Europe and the United States
Why the study?
Does the new liquid homogeneous assay for HDL-C provide reliable and accurate measurements compared to existing methods?
Population
Samples tested in seven laboratories
Comparison
New liquid homogeneous assay for direct… vs Designated comparison method, old homogeneous…
Authors
Discussion
Loading...
Member takes
Implication
May support consistent HDL-C testing in labs; confirms analytical reliability but leaves open effects on CHD risk assessment.
Structured PICO
Does the new liquid homogeneous assay for HDL-C provide reliable and accurate measurements compared to existing methods?
P
Population
Samples tested in seven laboratories
I
Intervention
New liquid homogeneous assay for direct measurement of high density lipoprotein cholesterol (HDL-C Plus)
C
Comparator
Designated comparison method, old homogeneous assay using lyophilized reagents, and other homogeneous and precipitation assays
O
Outcome
Assay performance including total CVs, bias, total error, and correlation with other methodssurrogate
The new liquid homogeneous HDL-C assay is reliable, easy to handle, and produces consistent results across laboratories, enabling accurate risk assessment for coronary heart disease.
Limitations
- Discrepant results in samples of patients with paraproteinemia
- Slight deviations observed in icteric samples
Cite This Study
Nauck et al. (1999) studied this question.