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The cell envelope of Enterobacteriaceae consists of membranes separated by a peptidoglycan layer. have been developed to separate the cytoplasmic from the outer membrane. outer membrane of Escherichia coli contains 60% the envelope protein. Using polyacrylamide, Schnaitman found six protein in the outer membrane fraction, of which one (mol. wt 44 000) accounted for 70% of the total membrane protein.
Lugtenberg et al. (Wed,) studied this question.
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