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October 15, 1975FEBS LettersOpen Access

Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bands

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Authors

BLBen LugtenbergTulane UniversityJMJos MeijersUtrecht UniversityRPR.C. PetersAgricultural Research Service

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Implication

Demonstrates the separation of outer membrane proteins in E. coli, suggesting significant protein composition insights.

Key Points

  • To investigate the electrophoretic characteristics of the major outer membrane protein in E. coli K12.
  • Used polyacrylamide gel electrophoresis to analyze outer membrane proteins.
  • Separated cytoplasmic and outer membranes to identify protein distribution.
  • Identified six protein bands in the outer membrane fraction.
  • One protein band (molecular weight 44,000) accounted for 70% of the total outer membrane protein.
  • Found six distinct protein bands in the outer membrane through electrophoresis.

Cite This Study

Lugtenberg et al. (1975) studied this question.

synapsesocial.com/papers/6a22a908cce2ba38c0cd5535https://doi.org/10.1016/0014-5793(75)80272-9
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