In Vitro Cultivation of Human Tumors: Establishment of Cell Lines Derived From a Series of Solid Tumors2

SUMMARY-A series of 200 human tumors were cultivated in vitro in an attempt to es-tablish cell lines. Lines were established with explant and trypsinization techniques, fr~m 13 tumors including ~arcinomas, sarcomas, mel-anomas, and brain tumors. All these lines in culture for over 1 year, exhibited marked refractility, multilayering, and criss-crossing and were morphologically distinct from nor-mal contact-inhibited human fibroblast or epithelial lines. They also formed colonies on IT!0nolay.e~s of n.ormal cells and grew with a high efficiency In soft agar. Preliminary re-sults!ndica~ed abnormal chromosomal pat-terns In all lines tested, and 8 of 9 cell lines