Cultured cells of the renin lineage maintained the memory to express the renin gene, which could be reenacted by cAMP and chromatin remodeling at the cAMP-responsive element.
Developed an in vitro model demonstrating that renin lineage cells maintain the memory to express the renin gene, which can be reactivated by cAMP and chromatin remodeling.
The renin-angiotensin system (RAS) regulates blood pressure and fluid-electrolyte homeostasis. A key step in the RAS cascade is the regulation of renin synthesis and release by the kidney. We and others have shown that a major mechanism to control renin availability is the regulation of the number of cells capable of making renin. The kidney possesses a pool of cells, mainly in its vasculature but also in the glomeruli, capable of switching from smooth muscle to endocrine renin-producing cells when homeostasis is threatened. The molecular mechanisms governing the ability of these cells to turn the renin phenotype on and off have been very difficult to study in vivo. We, therefore, developed an in vitro model in which cells of the renin lineage are labeled with cyan fluorescent protein and cells actively making renin mRNA are labeled with yellow fluorescent protein. The model allowed us to determine that it is possible to culture cells of the renin lineage for numerous passages and that the memory to express the renin gene is maintained in culture and can be reenacted by cAMP and chromatin remodeling (histone H4 acetylation) at the cAMP-responsive element in the renin gene.
Pentz et al. (Sat,) conducted a other in Renin-angiotensin system regulation. cAMP and chromatin remodeling was evaluated on Renin gene expression. Cultured cells of the renin lineage maintained the memory to express the renin gene, which could be reenacted by cAMP and chromatin remodeling at the cAMP-responsive element.
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