The encephalitis EV71 isolate exhibited 100-fold higher viral yields in astrocytoma cells at 96 hours post-infection compared to the herpangina EV71 isolate.
Does the EV71 isolate from encephalitis patients exhibit different cellular tropism and replication capacity compared to the isolate from herpangina patients?
Encephalitis-associated EV71 isolates demonstrate greater replication capacity in PBMCs and astrocytes and temperature resistance compared to isolates without CNS involvement.
Effect estimate: 100-fold higher
The cellular-tropism and biological characteristics of enterovirus 71 (EV71) isolates in Taiwan (TW) were studied. Growth curve experiments were conducted using cell lines that were possibly exhibited pathogenesis, and RT-PCR and sequencing tests were undertaken to amplify the 5' non-coding region (5'-NCR). The encephalitis isolate EV71 TW98NTU2078 was PBMC-tropic, temperature-resistant (Tr) at 40 degrees C, and easier to replicate in HTB-14 (astrocytoma) than the herpangina isolate EV71 TW98NTU1186 (The viral yields were 100-fold higher than those of the herpangina isolate EV71 TW98NTU1186 at 96 hr post infection.). The herpangina isolate EV71 TW98NTU1186 was non-PBMC-tropic, and temperature-sensitive (Ts) at 40 degrees C. The replication of EV71 TW98NTU1186 in HTB-14 was lower. No EV71 isolate infected HTB-37 (human colon adenocarcinoma cells). The encephalitis EV71 isolate exhibited better replication and transmission in PBMCs and astrocytes than did the EV71 isolate without CNS involvement.
Kung et al. (Tue,) conducted a other in Enterovirus 71 (EV71) infection. Encephalitis EV71 isolate (EV71 TW98NTU2078) vs. Herpangina EV71 isolate (EV71 TW98NTU1186) was evaluated on Viral yield in HTB-14 (astrocytoma) cells at 96 hr post infection (100-fold higher). The encephalitis EV71 isolate exhibited 100-fold higher viral yields in astrocytoma cells at 96 hours post-infection compared to the herpangina EV71 isolate.