Why the study?
Does the EV71 isolate from encephalitis patients exhibit different cellular tropism and replication capacity compared to the isolate from herpangina patients?
Population
Enterovirus 71 isolates obtained from patients with encephalitis and herpangina in Taiwan
Comparison
Infection of cell lines and temperature… vs Comparison between encephalitis isolate and…
Design
Preclinical
Follow-up
96 hours post infection
Key result
The encephalitis EV71 isolate exhibited 100-fold higher viral yields in astrocytoma cells at 96 hours post-infection compared to the herpangina EV71 isolate.
Authors
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Hypothesis-generating for EV71 neurovirulence; leaves open relevance to human encephalitis.
Does the EV71 isolate from encephalitis patients exhibit different cellular tropism and replication capacity compared to the isolate from herpangina patients?
Effect estimate: 100-fold higher
Encephalitis-associated EV71 isolates demonstrate greater replication capacity in PBMCs and astrocytes and temperature resistance compared to isolates without CNS involvement.
Kung et al. (2006) studied Enterovirus 71 (EV71) infection. Encephalitis EV71 isolate (EV71 TW98NTU2078) vs. Herpangina EV71 isolate (EV71 TW98NTU1186) was evaluated on Viral yield in HTB-14 (astrocytoma) cells at 96 hr post infection (100-fold higher). The encephalitis EV71 isolate exhibited 100-fold higher viral yields in astrocytoma cells at 96 hours post-infection compared to the herpangina EV71 isolate.
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