MPO-null mice experienced 35.1% less LV dilation (P<0.001) and a 52.2% improvement in LV function (P<0.0001) compared with wild-type mice, with no difference in infarct size.
Does myeloperoxidase deficiency improve left ventricular remodeling and reduce infarct size in murine models of myocardial infarction?
Myeloperoxidase-generated oxidants adversely affect left ventricular remodeling and function after myocardial infarction without significantly affecting infarct size.
p-value: p=<0.001
BACKGROUND: Inflammation after myocardial infarction (MI) heralds worse left ventricular (LV) function and clinical outcomes. However, whether inflammation affects LV function by extending myonecrosis and/or altering LV remodeling remains unknown. We hypothesized that cytotoxic aldehydes generated during oxidative stress may adversely affect remodeling and infarct size. One theoretical source of reactive aldehydes is oxidation of common alpha-amino acids by myeloperoxidase (MPO) released by leukocytes. However, a role for MPO in formation of aldehydes in vivo and the functional consequences of MPO-generated oxidants in ischemia/reperfusion models of MI have not been established. METHODS AND RESULTS: In studies with cell types found in vascular tissue, MPO-oxidation products of glycine (formaldehyde) and threonine (acrolein) were the most cytotoxic. Mass spectrometry studies of myocardial tissue from murine models of acute MI (both chronic left anterior descending coronary artery ligation and ischemia/reperfusion injury) confirmed that MPO serves as a major enzymatic source in the generation of these cytotoxic aldehydes. Interestingly, although MPO-null mice experienced 35.1% (P<0.001) less LV dilation and a 52.2% (P<0.0001) improvement in LV function compared with wild-type mice 24 days after ischemia/reperfusion injury, no difference in infarct size between wild-type and MPO-null mice was noted. CONCLUSIONS: The present data separate inflammatory effects on infarct size and LV remodeling and demonstrate that MPO-generated oxidants do not significantly affect tissue necrosis after MI but rather have a profound adverse effect on LV remodeling and function.
Vasilyev et al. (Tue,) conducted a other in Myocardial Infarction. MPO-null vs. Wild-type mice was evaluated on LV dilation, LV function, and infarct size (p=<0.001). MPO-null mice experienced 35.1% less LV dilation (P<0.001) and a 52.2% improvement in LV function (P<0.0001) compared with wild-type mice, with no difference in infarct size.
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