The automated Diasorin Liaison method for measuring plasma renin concentration yielded 100% sensitivity and 93% specificity for identifying primary aldosteronism compared to the conventional assay.
Cross-Sectional (n=225)
Does the automated PRC assay provide comparable diagnostic accuracy to the conventional PRA assay for primary aldosteronism screening?
The automated PRC assay is a feasible and accurate replacement for the conventional manual PRA assay in calculating the aldosterone/renin ratio for primary aldosteronism screening.
Effect estimate: 100% sensitivity, 93% specificity
BACKGROUND: Primary aldosteronism (PA), the most common secondary cause of hypertension, can be screened for using the aldosterone/renin ratio. This ratio is raised in PA and its accuracy depends on the ability to measure plasma renin at extremely low concentrations. METHODS: We compared two different procedures for assessing plasma renin. The conventional method, which measures plasma renin activity (PRA), is technically demanding and laborious, and the Diasorin Liaison method, which measures plasma renin concentration (PRC), is an automated immunoassay. Results from each method were used to calculate the aldosterone/renin ratio (ARR) and the performance of the Diasorin Liaison method compared with that of the conventional assay using receiver operator characteristic curves. RESULTS: The analytical and functional sensitivity of the PRC method were 2.1 and 5 microIU/mL, respectively. Intra- and inter-assay precision were 1.0 ng/mL/h showed significant correlation with PRC (r = 0.93; P 35, in patients with aldosterone >300 pmol/L, resulted in 100% sensitivity and 93% specificity, when compared with the commonly accepted aldosterone (pmol/L)/PRA (ng/mL/h) ratio of >750, in identifying patients who may suffer from PA. CONCLUSION: This study indicates the feasibility of using the automated PRC assay as a replacement for the conventional manual PRA assay in calculating the ARR as a first-line screen for PA.
Dorrian et al. (Wed,) conducted a cross-sectional in Primary aldosteronism (n=225). Diasorin Liaison method (plasma renin concentration) vs. Conventional method (plasma renin activity) was evaluated on Identification of patients who may suffer from primary aldosteronism using aldosterone/PRC ratio >35 vs aldosterone/PRA ratio >750 (100% sensitivity, 93% specificity). The automated Diasorin Liaison method for measuring plasma renin concentration yielded 100% sensitivity and 93% specificity for identifying primary aldosteronism compared to the conventional assay.