What question did this study set out to answer?

To establish a highly sensitive and rapid microplate ELISA capable of selectively quantifying angiotensin I in human biological fluids.

January 1, 1990Clinical and Experimental Hypertension30 citations

Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

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FSFumihiko Suzuki SYShinji Yamashita ATAkihiko Takahashi

Key Points

To establish a highly sensitive and rapid microplate ELISA capable of selectively quantifying angiotensin I in human biological fluids.
Developed a microplate ELISA utilizing an angiotensin I-peroxidase conjugate and specific anti-angiotensin I antibodies.
Employed 3,3',5,5'-tetramethylbenzidine as a substrate for spectrophotometric detection across human plasma and abdominal fluid samples.
Quantified angiotensin I levels down to a lower limit of detection of 1 pg.
Achieved selective antibody discrimination of angiotensin I from structurally related angiotensin II and angiotensin III.
Successfully validated the rapid assay system for measuring angiotensin I concentrations in human plasma and abdominal fluid.

Abstract

A microplate-ELISA was established for angiotensin (Ang) I using Ang I-peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3', 5,5'-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.

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Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

Key Points

Abstract

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