Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

Fumihiko Suzuki; Shinji Yamashita; Akihiko Takahashi; M. Ito; Seiji Miyazaki; Yukio NAGATA; Yukio Nakamura

doi:10.3109/10641969009074721

January 1, 1990Clinical and Experimental Hypertension

Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

View at Publisher

Ask AI

Bookmark

Share

Key Points

To establish a highly sensitive and rapid microplate ELISA capable of selectively quantifying angiotensin I in human biological fluids.
Developed a microplate ELISA utilizing an angiotensin I-peroxidase conjugate and specific anti-angiotensin I antibodies.
Employed 3,3',5,5'-tetramethylbenzidine as a substrate for spectrophotometric detection across human plasma and abdominal fluid samples.
Quantified angiotensin I levels down to a lower limit of detection of 1 pg.
Achieved selective antibody discrimination of angiotensin I from structurally related angiotensin II and angiotensin III.
Successfully validated the rapid assay system for measuring angiotensin I concentrations in human plasma and abdominal fluid.

Structured PICO

P

Population

Human plasma and abdominal fluid

I

Intervention

Microplate-ELISA for angiotensin I using Ang I-peroxidase conjugate, antiAng I antibodies, and 3,3',5,5'-tetramethylbenzidine

O

Outcome

Quantification of Angiotensin Isurrogate

Ask about this paper

Answers are grounded in this paper and cite their sources.

Implication

A highly sensitive and rapid microplate-ELISA was established for quantifying angiotensin I in human plasma and abdominal fluid.

Abstract

A microplate-ELISA was established for angiotensin (Ang) I using Ang I-peroxidase conjugate, antiAng I antibodies which could distinguish Ang I from Ang II and Ang III, and 3,3', 5,5'-tetramethylbenzidine. Ang I could be quantified in amounts as low as 1 pg by the spectrophotometric method. The present assay system was the most sensitive and rapid in all Ang I assay systems described so far. The assay system was applicable to determination of Ang I concentration in human plasma and abdominal fluid.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Suzuki et al. (1990) studied this question.

synapsesocial.com/papers/6a76223756c59a54388c5908 https://doi.org/10.3109/10641969009074721

View at Publisher

Ask AI

Bookmark

Share

Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Highly Sensitive Microplate-Elisa for Angiotensin I Using 3,3′,5,5′-Tetramethylbenzidine

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading