Key result
Novel angiotensin I enzyme immunoassay yields ~4.5-fold higher sensitivity than conventional RIA.
Why the study?
A more sensitive and specific assay for angiotensin I measurement was needed to improve plasma renin activity assessment.
Effect estimate: r = 0.94
A novel enzyme immunoassay for angiotensin I demonstrates higher sensitivity than standard RIA and correlates well for measuring plasma renin activity in dogs.
May enhance lab angiotensin I quantification; leaves open clinical adoption pending validation.
A very sensitive and specific enzyme immunoassay has been developed for angiotensin I. Angiotensin I was coupled to beta-D-galactosidase by a novel cross-linking reagent, N-(meta-maleimidobenzoyloxy)succinimide. No decrease in the enzyme activity was observed during the coupling procedure. In the angiotensin I-beta-D-galactosidase conjugate, 0.39 mol immunoreactive angiotensin I/mol enzyme were present. A competitive assay with the enzyme-labeled angiotensin I was performed. Antibody-bound and free labeled antigen were separated from each other by the second antibody method, and the enzyme activity of the former was estimated. Using this assay, angiotensin I could be detected in the range of 1.2--50 pg. The sensitivity was 4.5-fold higher than that of the usual RIA. This assay distinguished clearly angiotensin I from angiotensin II, angiotensin III, and (Sar1, Ile8)-angiotensin II. The present method was applied to measure PRA in dogs; the results correlated fairly well with those obtained by the RIA (r = 0.94).
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Aikawa et al. (1979) studied this question. Enzyme immunoassay for angiotensin I vs. Radioimmunoassay (RIA) was evaluated on Correlation of plasma renin activity measurements with RIA (r = 0.94). A newly developed enzyme immunoassay for angiotensin I demonstrated a sensitivity 4.5-fold higher than usual RIA and correlated well with RIA for measuring plasma renin activity in dogs (r = 0.94).
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