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SummaryMethods are described for producing high-titered group-specific antisera for classifying hemolytic streptococci into groups. A description is also given of a reliable micro-precipitin technic which can be used with very small amounts of bacterial extract and antiserum. Attention is called to the occurrence of some non-hemolytic members of Groups B, C, D, and G.Since this communication was submitted for publication, Fuller has reported a method of extracting streptococci with formamide.
Rebecca C. Lancefield (Sun,) studied this question.