Studies in glycolysis

IN a previous communication Kendal & Stickland, 1937, 1 it was shown that if rabbit muscle is minced and thoroughly washed with water, and then extracted several times with dilute Na2HPO4, the later extracts (usually the 4th) contain much of the glycolytic enzyme system but are freer from coenzymes than extracts prepared by earlier methods. This was shown by the fact that on addition of boiled muscle extract a rapid formation of lactic acid from poly- saccharide took place, while on addition of Mg+ +, adenosinetriphosphate, cozvmase and hexosediphosphate practically no lactic acid was formed. It was also shown that if Mg++ and adenosinetriphosphate alone were added to such extracts considerable quantities of phosphate were esterified, although no lactic acid was formed. The present communication deals with the nature of the esters formed under these conditions.