A noninvasive Doppler method successfully measured arterial wall excursion (ranging from 13 microm in ApoE to 95 microm in alphaSMA mice) and augmentation index across different mouse models.
A newly developed noninvasive Doppler method can successfully measure arterial wall motion and identify characteristic changes in arterial properties associated with age and atherosclerosis in mouse models.
To facilitate assessment of arterial function, we developed a noninvasive Doppler method for measuring vessel motion in genetically altered mice. A 20 MHz probe was held by an alligator clip and positioned over the carotid arteries of 16 mice including six 3 to 5-month old wild-type (WT), four 30-month old senescent (Old), two apolipoprotein-E (ApoE), and four alpha smooth muscle actin (alphaSMA) mice. Doppler signals were obtained simultaneously from both vessel walls and from blood flow using one or two probes. The displacement signals from the near and far walls were subtracted to generate a diameter signal from which the excursion and an augmentation index were calculated. The excursion ranged between 13 microm (in ApoE) and 95 microm (in alphaSMA). The augmentation index was lowest in the WT mice (0.06) and highest in the Old mice (0.29). This noninvasive method is able to identify and confirm characteristic changes in arterial properties associated with age, atherosclerosis, and the absence of vascular tone.
Hartley et al. (Tue,) conducted a other in Arterial function assessment in genetically altered mice (n=16). Noninvasive Doppler method for measuring vessel motion was evaluated on Excursion and augmentation index of arterial wall motion. A noninvasive Doppler method successfully measured arterial wall excursion (ranging from 13 microm in ApoE to 95 microm in alphaSMA mice) and augmentation index across different mouse models.
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