July 4, 2018Open Access

A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice

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Abstract

Our comprehensive and rigorous analysis of WGS data across multiple sample types suggests both Cas9 and Cpf1 nucleases are very specific in generating targeted DNA modifications and off-targeting can be avoided by designing guide RNAs with high specificity.

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Tang et al. (Wed,) studied this question.

synapsesocial.com/papers/69d9798f94760e72e6a3c9c6 https://doi.org/https://doi.org/10.1186/s13059-018-1458-5

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