Trigonelline induces browning in 3T3-L1 white adipocytes and improves lipid metabolism by activating β3-AR and inhibiting PDE4, stimulating the p38 MAPK/ATF-2 signaling pathway.
Does trigonelline induce browning and improve lipid metabolism in 3T3-L1 white adipocytes?
Trigonelline induces browning and improves lipid metabolism in 3T3-L1 white adipocytes, suggesting potential as an anti-obesity compound.
Trigonelline, a major alkaloid component of fenugreek, has been demonstrated to have several biological activities, including antidiabetic and anticancer effects. This study aimed to examine the possible application of trigonelline as an anti-obesity compound based on an investigation of its enhancement of lipid catabolism and induction of browning in white adipocytes. Trigonelline induces browning of 3T3-L1 white adipocytes by enhancing the expressions of brown-fat signature proteins and genes as well as beige-specific genes, including Cd137, Cited1, Tbx1, and Tmem26. Trigonelline also improves lipid metabolism in white adipocytes by decreasing adipogenesis and lipogenesis as well as promotes lipolysis and fatty acid oxidation. Moreover, trigonelline increases the expression of Cox4, Nrf1, and Tfam genes that are responsible for mitochondrial biogenesis. Mechanistic studies revealed that the browning effect of trigonelline in 3T3-L1 white adipocytes is mediated by activating β3-AR and inhibiting PDE4, thereby stimulating the p38 MAPK/ATF-2 signaling pathway. Considering its high bioavailability in humans and the results of this study, trigonelline may have potential as an anti-obesity compound.
Choi et al. (Mon,) conducted a other in Obesity. Trigonelline was evaluated on Browning of 3T3-L1 white adipocytes and lipid metabolism. Trigonelline induces browning in 3T3-L1 white adipocytes and improves lipid metabolism by activating β3-AR and inhibiting PDE4, stimulating the p38 MAPK/ATF-2 signaling pathway.