Isoprenaline 150 mg/kg induced Takotsubo syndrome in female rats, increasing plasma interleukin-1β and activating necroptosis and pyroptosis pathways at 24 hours.
Does isoprenaline-induced Takotsubo syndrome activate inflammatory-associated programmed cell death (necroptosis and pyroptosis) in female rats?
In a rat model of Takotsubo syndrome, isoprenaline induces inflammatory-associated programmed cell death, including necroptosis and pyroptosis.
Takotsubo syndrome (TTS) is associated with inflammatory response, therefore the aim of the study was to evaluate the presence and dynamics of inflammatory-associated forms of cell death, necroptosis, and pyroptosis in the female rat model of isoprenaline (ISO)-induced TTS. TTS was induced in female Sprague Dawley rats (n = 36) by ISO 150 mg/kg intraperitoneally. Animals were divided into four groups: TTSO (TTS+ovariectomy; n = 10), TTSP (TTS+sham operation; n = 10), CO (0.9% NaCl+ovariectomy; n = 8), CP (0.9% NaCl+sham operation; n = 8). Histopathological analysis, evaluation of plasma concentration, and myocardial expression of pyroptosis- and necroptosis-associated proteins were performed. TTSO and TTSP groups had higher plasma concentrations of interleukin-1β in comparison with the controls. Low myocardial protein expression of mixed lineage kinase domain-like pseudokinase (MLKL), caspase-1 (Casp-1), and calcium/calmodulin-dependent kinase type II isoform delta (CAMKIIδ) was visible 6 and/or 12 h post-ISO. Twenty-four hours post-ISO, high myocardial and vascular protein expression of CAMKIIδ was visible in TTSO but not TTSP rats, while high myocardial expression of MLKL and Casp-1 was visible both in TTSO and TTSP rats. The course of TTS is associated with activation of inflammatory-associated programmed cell death, necroptosis, and pyroptosis, therefore inflammation may be a primary response occurring simultaneously with cardiomyocyte death in TTS.
Borodzicz-Jażdżyk et al. (Fri,) conducted a other in Isoprenaline-induced Takotsubo syndrome (n=36). Isoprenaline vs. 0.9% NaCl was evaluated on Presence and dynamics of inflammatory-associated forms of cell death, necroptosis, and pyroptosis. Isoprenaline 150 mg/kg induced Takotsubo syndrome in female rats, increasing plasma interleukin-1β and activating necroptosis and pyroptosis pathways at 24 hours.
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