Luminosa: making confocal fluorescence microscopy a tool for every biophysicist

Quantitative time-resolved fluorescence techniques like FLIM are increasingly employed in fields like phase separation and cellular sensing. PicoQuant`s new microscope Luminosa combines state-of-the-art hardware with cutting edge software, delivering high quality data while simplifying daily operation. The software includes features like context-based workflows, sample-free auto-alignment and laser power calibration which improve reproducibility of experiments. We describe how FLIM is streamlined with Luminosa. Its rapidFLIM hardware records several frames per second with high count rates, which the software handles with a novel dynamic binning format. Combined with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images with minimal user interaction. We will also show an outlook about how Luminosa can be used for combining FLIM with super-resolution modalities.