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This protocol is used to extract genomic DNA from 0.22μl sterivex filters using Phenol-Chlorophorm. As part of the Hakai Institute Ocean Observing Program, biomolecular samples have been collected weekly, from 0 m to near bottom (260 m), to genetically characterize plankton communities in the Northern Salish Sea since 2015. This protocol is developed to work across all domains of life, from viruses to prokaryotes to eukaryotes, allowing for both amplicon sequencing and shotgun sequencing.
Colleen Kellogg (Mon,) studied this question.
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