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One hundred samples of patients with conjunctivitis who visited the Fallujah Teaching Hospital's eye consultation were included in the study. For both genders, the age range covered was 9 months to 77 years. The study found that 87% of the samples were positive for bacterial growth, while 13% were negative for it. 56.32% of them were for males and 43.67% for females. A variety of bacteria were isolated, including Staphylococcus aureus, Staph-epidermidis, and E. coli. The study demonstrates that the minimum inhibitory concentration (MIC) of each black tea aqueous extract and vancomycin was 12.5, which gives 100% inhibition. The inhibition effects of synergistic black tea extract and vancomycin were at concentrations of 6.2 giving 100% inhibition. Also, black tea extract has a beneficial effect on the gene expression of the 16S rRNA gene of E. coli bacteria in cooperation with untreated samples, which is down-regulation. Furthermore, the black tea extract affected all types of Staph-epidermidis bacteria by increasing their gene expression (upregulation) in cooperation with the untreated sample
halboosi et al. (Sat,) studied this question.