Single-Molecule Studies of Small Molecule Drugs through Nanopore Detection

During the last decades, nanopore single-molecule analysis has shown great potential in detection of DNA 1-3, RNA 4, proteins 5- 8, polymers 9, organic molecules 10-11, and chemical reactions 12-13, and has been further extended to high-throughput nucleic acid sequencing. The detection of drug interactions 14 is crucial to evaluate the effect and safety of drugs, and it is also an indispensable part in the process of drug development and clinic treatment 15. Over the past few years, the nanopore worked as a new platform for the discovery of drug interactions. Nanopore sensor, with the advantages of simple operation, speed and portability, can allow single molecule to pass through.