Conductive Mesh Electrodes for Electrochemical Biosensors

firstₚage Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Font Size: Aa Aa Aa Line Spacing:    Column Width:    Background: Open AccessAbstract Conductive Mesh Electrodes for Electrochemical Biosensors † by Mohamed SharafeldinMohamed Sharafeldin SciProfiles Scilit Preprints. org Google Scholar Department of Chemistry, University of Otago, P. O. Box 56, Dunedin 9054, New Zealand † Presented at the 4th International Electronic Conference on Biosensors, 20–22 May 2024; Available online: https: //sciforum. net/event/IECB2024. Proceedings 2024, 104 (1), 1; https: //doi. org/10. 3390/proceedings2024104001 Published: 28 May 2024 (This article belongs to the Proceedings of The 4th International Electronic Conference on Biosensors) Download keyboardₐrrowdown Download PDF Download PDF with Cover Download XML Download Epub Versions Notes Keywords: biosensors; electrochemical assay; biomarker detection; mesh electrodes; conductive polymers 1. IntroductionThe rate of recruiting a target analyte on a receptor-functionalized electrode is a limiting step in most electrochemical biosensors, and is predominantly diffusion-controlled. Enhancing the interaction dynamics between the target analyte and the electrode-immobilized receptors offer great potential for improving electrochemical assay parameters in terms of assay time and sensitivity. Nanostructured electrodes, such as those modified with nanoparticles, can enhance the functional surface area; however, they have a limited ratio of sample volume to electrode surface area. 2. MethodsWe propose the use of highly conductive mesh electrodes as a solid support for the immobilization of receptors (using antibodies as a model) and apply this in an electrochemical sensor that can be used for the detection of a range of target species. This approach involves coating commercially available mesh supports with low-fouling conductive polymers, which can be further modified with bio-receptors such as antibodies, proteins, or aptamers. 3. Results and ConclusionsThese functionalized mesh electrodes are employed for the isolation and detection of target biomarkers. The system is integrated within a 3D-rpinted microfluidic chip to allow real-time isolation and detection of the analytes under continuous flow using impedimetric, voltammetric, or amperometric assays. This integrated system allows for high target recovery within the mesh-like structures, overcoming the mass transport limitations associated with conventional disc or screen-printed electrode-based electrochemical assays. Additionally, this system is applied for dual-mode target isolation, where the mesh electrode enables size-based exclusion of the target analyte, thereby improving assay specificity. This method significantly boosts the ratio of sample volume to surface area (approximately a tenfold increase compared to disc electrodes), facilitating a more effective interaction between target analytes and receptors immobilized on the electrode. This augmentation is bolstered by the flow-through format of the system, as opposed to the traditional sample incubation method used with disc electrodes. FundingThis work was supported by University of Otago research grant #120543. Institutional Review Board StatementNot applicable. Informed Consent StatementNot applicable. Data Availability StatementData associated with this work can be obtained by contacting the author. Conflicts of InterestThe author declares no conflict of interest. Disclaimer/Publisher's Note: The statements, opinions and data contained in all publications are solely those of the individual author (s) and contributor (s) and not of MDPI and/or the editor (s). MDPI and/or the editor (s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. © 2024 by the author. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https: //creativecommons. org/licenses/by/4. 0/). Share and Cite MDPI and ACS Style Sharafeldin, M. Conductive Mesh Electrodes for Electrochemical Biosensors. Proceedings 2024, 104, 1. https: //doi. org/10. 3390/proceedings2024104001 AMA Style Sharafeldin M. Conductive Mesh Electrodes for Electrochemical Biosensors. Proceedings. 2024; 104 (1): 1. https: //doi. org/10. 3390/proceedings2024104001 Chicago/Turabian Style Sharafeldin, Mohamed. 2024. "Conductive Mesh Electrodes for Electrochemical Biosensors" Proceedings 104, no. 1: 1. https: //doi. org/10. 3390/proceedings2024104001 Article Metrics No No Article Access Statistics Multiple requests from the same IP address are counted as one view.