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This protocol outlines the steps for detecting multiple viral gene targets for the pathogens SARS-CoV-2, CrAssphage and HEV using a qPCR assay. Total Nucleic Acid (TNA) extracted from environmental samples (wastewater) is used for testing. Key Steps and Considerations: qPCR Amplification Cycles: Follow the outlined cycling conditions for optimal amplification of target viral RNA. 2. Target Detection: Detect SARS-CoV-2 and HEV using specific primers and probes designed for these targets.
Abraham et al. (Fri,) studied this question.
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