Key points are not available for this paper at this time.
The following protocol is optimized for the detection of Salmonella Paratyphi A and Vibrio cholerae using multiplex qPCR assays from TNA extracted from environmental samples (wastewater samples). Total Nucleic Acid (TNA) extracted from the pellet particles is used as template in a real-time PCR assay which uses primer-probes specific for Salmonella Paratyphi A or Vibrio cholerae gene targets. Three different multiplex PCR assays were designed following standardization and validation of the primer probe combinations.
Abraham et al. (Fri,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: