Figure 1 from Spatial Landscape of Malignant Pleural and Peritoneal Mesothelioma Tumor Immune Microenvironments

Profiling the spatial landscape of malignant mesothelioma using mIF. A, Schematic diagram outlining the acquisition of multiplexed immunofluorescence images from patients with malignant mesothelioma generated using BioRender.com. B, Waterfall plot depicting the distribution of cell populations as a percentage of all cells in the TIME, categorized by CD8+ T cells and sorted across histologic subgroups within MPM and MPeM. Vertical bars represent cell percentages, with distinct colors corresponding to various cell lineages. C, Pie charts depicting the mean cellular composition in samples with MPM and MPeM, including relative frequencies of B cells, CD4+ T cells, CD8+ T cells, Tregs, macrophages, DCs, NK cells, pan-CK+ tumor cells, and unidentified cells. D, Assessment of the prevalence of CD8+ T cells and pan-CK+ tumor cells in MPM and MPeM as a fraction of the total cell population. P values were calculated using the one-sided Mann–Whitney U test. RPCI, Roswell Park Cancer Institute; UPENN, University of Pennsylvania; UPITT, University of Pittsburgh.