Multiplex Nested PCR for Vibrio Cholera v1

Primers targeting various regions of the V. Cholera genome were obtained from the literature or designed, and optimized for a multiplex nested PCR assay. The following primers were extracted from Hoshino et al. , 1998: O1ᵣfbForInner (modified here by Dr Alex Shaw), O1ᵣfbRevInner, O139ᵣfbForInner, O139ᵣfbRevOuter. The following primers were obtained from Theron et al. , 2000: ctxA Inner Rev, ctxAᵣev. The following primers were obtained from Nandi et al. , 2000: ctxAFor, ompWFor, ompWRev, ompWInnerRev. The following primers were designed by Dr Alex Shaw and are unpublished: ToxRForOuter, ToxRevOuter, ToxRForInner, O1ᵣfbForOuter, O1ᵣfbRevOuter, O139ᵣfbForOuter, O139ᵣfbRevOuter.